aor 012  (Alomone Labs)

Journal: International Journal of Molecular Sciences

Article Title: Dose-Dependent Effect of Hyperbaric Oxygen Treatment on Burn-Induced Neuropathic Pain in Rats

doi: 10.3390/ijms20081951

Figure Lengend Snippet: ( a , b ) Immunohistochemical (IHC) results of melatonin receptor 1 (MT1), melatonin receptor 2 (MT2), μ (MOR) and κ (KOR) opioid receptor in cuneate nucleus of medulla oblongata. MT1, MT2, MOR, and KOR expression significantly increased in Burn 4w with HBOT 1w compared with Burn 4w with sham HBOT 1w. The expression of MT1, MT2, MOR and KOR were not upregulated after two-weeks HBOT. (*** p < 0.001, original magnification: 200×, w = weeks, SB = sham burn, SH = sham HBOT, MO = medulla oblongata, CN = cuneate nucleus).

Article Snippet: After thawing at room temperature for 30 min, sections were incubated separately with anti-brain-derived neurotrophic factor (BDNF) (1:100; Novus Biologicals, LLC, Centennial, CO, USA), anti-glial fibrillary acidic protein (GFAP) (1:500; Abcam, Cambridge, UK), anti-melatonin 1A (1:100; Sigma-Aldrich, Merck KGaA), anti-melatonin 1B(1:100; Abcam), anti-MOR(1:500; Alomone Labs, Jerusalem), or anti-KOR(1:500; Alomone Labs).

Techniques: Immunohistochemical staining, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Dose-Dependent Effect of Hyperbaric Oxygen Treatment on Burn-Induced Neuropathic Pain in Rats

doi: 10.3390/ijms20081951

Figure Lengend Snippet: ( a , b ) Immunohistochemical (IHC) results of MT1, MT2, MOR, and KOR in the right dorsal horn of the spinal cord. MT1, MT2, MOR, and KOR expression significantly increased in Burn 4w with HBOT 1w compared with Burn 4w with sham HBOT 1w. The expression of MT1, MT2, MOR and KOR were not upregulated after two-weeks HBOT. ( c ) RT-PCR results of MT1 and MT2 relative quantification. The mRNA expression significantly increased in Burn 4w with HBOT 1w compared with Burn 4w with sham HBOT 1w. The mRNA expression increased in Burn 4w with HBOT 2w compared with Burn 4w with sham HBOT 2w. (*** p < 0.001, original magnification: 200×, w = weeks, SB = sham burn, SH = sham HBOT).

Article Snippet: After thawing at room temperature for 30 min, sections were incubated separately with anti-brain-derived neurotrophic factor (BDNF) (1:100; Novus Biologicals, LLC, Centennial, CO, USA), anti-glial fibrillary acidic protein (GFAP) (1:500; Abcam, Cambridge, UK), anti-melatonin 1A (1:100; Sigma-Aldrich, Merck KGaA), anti-melatonin 1B(1:100; Abcam), anti-MOR(1:500; Alomone Labs, Jerusalem), or anti-KOR(1:500; Alomone Labs).

Techniques: Immunohistochemical staining, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: International Journal of Molecular Sciences

Article Title: Dose-Dependent Effect of Hyperbaric Oxygen Treatment on Burn-Induced Neuropathic Pain in Rats

doi: 10.3390/ijms20081951

Figure Lengend Snippet: One-week HBOT upregulated MT1, MT2, MOR, and KOR expression in the cuneate nucleus of medulla oblongata and in the right dorsal horn of the spinal cord. HBOT downregulated BDNF in the right dorsal horn and Substance P and CGRP in right hind paw skin. HBOT alleviated burn-induced neuropathic pain via this pain transmission pathway (MO = medulla oblongata, CN = cuneate nucleus).

Article Snippet: After thawing at room temperature for 30 min, sections were incubated separately with anti-brain-derived neurotrophic factor (BDNF) (1:100; Novus Biologicals, LLC, Centennial, CO, USA), anti-glial fibrillary acidic protein (GFAP) (1:500; Abcam, Cambridge, UK), anti-melatonin 1A (1:100; Sigma-Aldrich, Merck KGaA), anti-melatonin 1B(1:100; Abcam), anti-MOR(1:500; Alomone Labs, Jerusalem), or anti-KOR(1:500; Alomone Labs).

Techniques: Expressing, Transmission Assay